Development of Quality Control Parameters for Standardization of Herb of the Cultivated Plant <i>Alchemilla mollis</i> (Buser) Rothm.

Introduction. Alchemilla herb is proposed for introduction into pharmaceutical practice, obtaining extracts with various pharmacological activity. To expand, resource base of it has been to use a high biomass cultivated plant – mollis (Buuser) Rothm. In terms qualitative and quantitative characteristics the chemical composition comparable samples raw materials wild-growing . The presence chemotaxonomic features necessitates preparation regulatory documentation this as source medicinal materials. Aim. Development methodology assessing main group biologically active substances determination flavonoids in herb. Materials methods. As objects study, we used harvested from plants Perm Krai. chromatographic parameters material authenticity were determined by thin layer chromatography (TLC). develop herb, modification method was carried out. Results discussion. During study identified cinaroside, rutin, quercetin. Cynaroside referred marker substances. Ethyl acetate : acetic acid (85 15) chosen optimal system. A validation change extractant, particle size, time frequency extraction sample cynaroside standard substance are substantiated. conditions reaction complex formation aluminum chloride established. Conclusion. determine identification TLC. modified reproducible, correct can be standardization. When testing on region, range values content 3.14–4.84 %, an average level variability.